A Brisbane nightclub has defended its controversial decision to ban all high-profile footballers from entering, citing recent incidents involving rugby league players.

Uber at West End has introduced a policy of refusing entry to all professional football players from any team or code because of recent media attention surrounding the behaviour of players at local venues.

Two alleged incidents at Uber involving NRL players, including Cronulla's Greg Bird and North Queensland's Sione Faumuina , had contributed to the decision, venue owner Jim Davies said.

Three Brisbane Broncos players have been linked to an alleged sexual assault of a woman in the men's toilet of Fortitude Valley nightspot Alhambra Lounge on Saturday night.

"Due to recent events that had been widely reported, Uber is trialling a policy of not allowing high-profile footballers into the venue," Mr Davies said.

"The position of the company is to provide a safe and welcoming environment for all patrons, whether or not they have a public profile."

The ban would not be difficult to enforce because security staff knew what most local professional players looked like, he said.

Mr Davies denied the policy discriminated on the basis of employment and said any patron could be refused entry or ejected from the venue for bad behaviour.

The ban would probably have little impact on football players because the majority chose to party at other Brisbane venues, he said.

"Uber has been portrayed as a football venue, but we're certainly not ... we're not a place footballers would normally come to," he said.

"We have had footballers in here in the past but they don't come on a regular basis.

"I think the media has damaged the reputation of Uber more than the footballers in some instances."

Mr Davies refused to comment on the behaviour of football players at Uber in the past, including media reports that Broncos star Karmichael Hunt was recently refused entry.

"I'm not saying there's a problem with football players but this position is due to the high media profile at the moment.

"We don't need any adverse publicity ... we are acting in accordance with the law."

The effectiveness of the policy would be reviewed in the future, he said.